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The A3243G mutation in the human mitochondrial tRNALeu(UUR) gene causes a . proteins have a half-life of over 100 h (Hare and Hodges 1982; Grisolia et al. .. miniprep kit (Qiagen) including treatment with RNase-free DNase (Qiagen). . Attardi G. MELAS mutation in mtDNA binding site for transcription termination
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Jan 15, 2003 Indeed, molecular‐level studies of hs mt tRNA Leu(UUR) and tRNA Ile have revealed To date, 18 pathogenic mutations have been identified within the gene The anticodon stem of this mutant appears to be completely unstructured. . but is more distant from the site of the pathogenic mutation than the
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